Emanuelsson B
J Chromatogr. 1978 Jul 1;146(1):113-7. doi: 10.1016/s0378-4347(00)81295-5.
A method is described for the quantitation of new non-tricyclic antidepressant, zimelidine, and its pharmacologically active, N-demethylated metabolite, norzimelidine, in plasma. The method involves a single extraction of basified plasma with diethyl ether, concentration of the ethereal extract, chromatography on a high-performance liquid chromatograph and quantitation using a variable-wavelength UV detector. The respective geometric isomers of zimelidene and norzimelidine are used as internal standards for quantitation. Resolution is effected using a 5-micron silica gel column with an aqueous methanolic solution of ammonium nitrate as the mobile phase. The minimum quantitated amount was 25 ng and the coefficient of variation for the method did not exceed 7% in the range 25 to 1000 ng/ml for both compounds. The method has been applied in monitoring the plasma concentration of zimelidine and norzimelidine in plasma from depressed patients and an example of this application is presented.
本文描述了一种定量测定血浆中新型非三环类抗抑郁药齐美利定及其药理活性N-去甲基代谢物去甲齐美利定的方法。该方法包括用乙醚对碱化血浆进行单次萃取，浓缩乙醚提取物，在高效液相色谱仪上进行色谱分析，并使用可变波长紫外检测器进行定量。齐美利定和去甲齐美利定各自的几何异构体用作定量的内标。使用5微米硅胶柱，以硝酸铵的甲醇水溶液为流动相进行分离。最低定量限为25纳克，两种化合物在25至1000纳克/毫升范围内该方法的变异系数均不超过7%。该方法已用于监测抑郁症患者血浆中齐美利定和去甲齐美利定的浓度，并给出了该应用的一个实例。
