Mix L L, Dinerstein R J, Villereal M L
Biochem Biophys Res Commun. 1984 Feb 29;119(1):69-75. doi: 10.1016/0006-291x(84)91619-x.
Intracellular free Ca+2 concentration was measured in cultured human fibroblasts (HSWP) utilizing the Ca+2-sensitive fluorescent probe quin2. The addition of peptide growth factors to serum-deprived HSWP cells induced an immediate rise in intracellular Ca+2 concentration. This mitogen-induced rise in Ca+2 concentration could be blocked by the addition of the intracellular Ca+2 antagonist TMB-8. Addition of the phospholipase activator, melittin, to cells in the absence of growth factors also caused a dramatic rise in intracellular Ca+2 concentration which was blocked by TMB-8.
利用钙离子敏感荧光探针喹啉-2（quin2）测量培养的人成纤维细胞（HSWP）中的细胞内游离钙离子浓度。向血清饥饿的HSWP细胞中添加肽生长因子会导致细胞内钙离子浓度立即升高。这种有丝分裂原诱导的钙离子浓度升高可通过添加细胞内钙离子拮抗剂TMB-8来阻断。在没有生长因子的情况下，向细胞中添加磷脂酶激活剂蜂毒素（melittin）也会导致细胞内钙离子浓度急剧升高，这一升高也被TMB-8阻断。
