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使用辛基葡糖苷将细胞色素P-450单加氧酶系统整合到大型单层脂质体中，特别是用于测量膜中蛋白质的扩散。

Incorporation of the cytochrome P-450 monooxygenase system into large unilamellar liposomes using octylglucoside, especially for measurements of protein diffusion in membranes.

作者信息

Schwarz D, Gast K, Meyer H W, Lachmann U, Coon M J, Ruckpaul K

出版信息

Biochem Biophys Res Commun. 1984 May 31;121(1):118-25. doi: 10.1016/0006-291x(84)90695-8.

DOI:10.1016/0006-291x(84)90695-8
PMID:6704207
Abstract

Cytochrome P-450 and NADPH cytochrome P-450 reductase were incorporated into large unilamellar lipid vesicles (200-300 nm in diameter) removing octylglucoside from mixed micelles by dialysis. The large size of the protein-containing liposomes guarantees a negligibly small vesicle tumbling. Such large vesicles are better suited for studies of protein rotation in reconstituted membranes than vesicles prepared by use of bile salts. At present the octyl-glucoside reconstituted monooxygenase system seems to be the most appropriate model for studying protein-protein and protein-lipid interactions in liver microsomes due to the similarity with respect to the main structural and functional properties, including size.

摘要

细胞色素P - 450和NADPH细胞色素P - 450还原酶被整合到直径为200 - 300纳米的大单层脂质囊泡中，通过透析从混合胶束中去除辛基葡糖苷。含蛋白质的脂质体尺寸较大，保证了囊泡翻滚的影响可忽略不计。与使用胆盐制备的囊泡相比，这种大囊泡更适合用于研究重组膜中蛋白质的旋转。目前，由于在包括大小在内的主要结构和功能特性方面具有相似性，辛基葡糖苷重组单加氧酶系统似乎是研究肝微粒体中蛋白质 - 蛋白质和蛋白质 - 脂质相互作用的最合适模型。

相似文献

1
Incorporation of the cytochrome P-450 monooxygenase system into large unilamellar liposomes using octylglucoside, especially for measurements of protein diffusion in membranes.使用辛基葡糖苷将细胞色素P-450单加氧酶系统整合到大型单层脂质体中，特别是用于测量膜中蛋白质的扩散。
Biochem Biophys Res Commun. 1984 May 31;121(1):118-25. doi: 10.1016/0006-291x(84)90695-8.
2
Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 1. Solubilization of large unilamellar liposomes (prepared by reverse-phase evaporation) by triton X-100, octyl glucoside, and sodium cholate.在涉及使用去污剂的重组过程中膜蛋白插入脂质体的机制。1. 用曲拉通X-100、辛基葡糖苷和胆酸钠溶解大单层脂质体（通过反相蒸发制备）。
Biochemistry. 1988 Apr 19;27(8):2668-77. doi: 10.1021/bi00408a006.
3
Effects of detergent on substrate binding and spin state of purified liver microsomal cytochrome P-450LM2 from phenobarbital-treated rabbits.去污剂对苯巴比妥处理的兔肝脏微粒体细胞色素P - 450LM2底物结合及自旋状态的影响。
Biochemistry. 1985 Jul 2;24(14):3809-14. doi: 10.1021/bi00335a058.
4
Reconstitution of the liver microsomal monooxygenase system in liposomes from dimyristoylphosphatidylcholine.
Biomed Biochim Acta. 1984;43(3):281-93.
5
Phospholipid bilayer membranes play decisive roles in the cytochrome P-450-dependent monooxygenase system.磷脂双分子层膜在细胞色素P - 450依赖的单加氧酶系统中起决定性作用。
J Cancer Res Clin Oncol. 1988;114(4):335-40. doi: 10.1007/BF02128175.
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Interaction between NADPH-cytochrome P-450 reductase and cytochrome P-450 in the membrane of phosphatidylcholine vesicles.烟酰胺腺嘌呤二核苷酸磷酸-细胞色素P-450还原酶与细胞色素P-450在磷脂酰胆碱囊泡膜中的相互作用。
Biochim Biophys Acta. 1979 Jan 19;550(2):341-56. doi: 10.1016/0005-2736(79)90220-7.
7
Interaction of hexane phosphonic acid diethyl ester with phospholipids in hepatic microsomes and reconstituted liposomes as studied by 31P-NMR.
Biochim Biophys Acta. 1985 Jan 25;812(2):437-46. doi: 10.1016/0005-2736(85)90318-9.
8
Preparation and properties of large octylglucoside dialysis/adsorption liposomes.大辛基葡糖苷透析/吸附脂质体的制备及性质
Biomed Biochim Acta. 1988;47(7):609-21.
9
Cholesterol metabolism by purified cytochrome P-450scc is highly stimulated by octyl glucoside and stearic acid exclusively in large unilamellar phospholipid vesicles.
Biochemistry. 1989 Oct 17;28(21):8397-402. doi: 10.1021/bi00447a019.
10
Hydrodynamic properties of monomeric cytochromes P-450LM2 and P-450LM4 in n-octylglucoside solution.
Biochem Biophys Res Commun. 1982 Jul 16;107(1):265-71. doi: 10.1016/0006-291x(82)91699-0.

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Kinetics of dithionite-dependent reduction of cytochrome P450 3A4: heterogeneity of the enzyme caused by its oligomerization.连二亚硫酸盐依赖性细胞色素P450 3A4还原反应的动力学：酶寡聚化导致的酶异质性
Biochemistry. 2005 Oct 25;44(42):13902-13. doi: 10.1021/bi0509346.
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Heterogeneity in rabbit liver cytochrome P-450 LM2 observed by cation exchange HPLC: partial biochemical characterization of the two major LM2 subfractions.通过阳离子交换高效液相色谱法观察到的兔肝细胞色素P-450 LM2的异质性：两种主要LM2亚组分的部分生化特性
Mol Cell Biochem. 1994 Dec 7;141(1):1-7. doi: 10.1007/BF00935584.
3
Determination of the partition of the tetramisole derivative (+-)-5,6-dihydro-6-phenyl-2-n-propyl-imidazo[2,1-b]thiazole into liposomal membranes by fluorescence quenching of the membrane probe 8-(2-anthryl)-octanoic acid.
通过膜探针8-(2-蒽基)-辛酸的荧光猝灭法测定四咪唑衍生物(±)-5,6-二氢-6-苯基-2-正丙基-咪唑并[2,1-b]噻唑在脂质体膜中的分配情况。
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