3-（三氟甲基）-3-（间-[¹²⁵I]碘苯基）重氮丙啶，一种疏水性光反应探针，以钙离子依赖的方式标记钙调蛋白和钙调蛋白片段。
3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, a hydrophobic, photoreactive probe, labels calmodulin and calmodulin fragments in a Ca2+-dependent way.
作者信息
Krebs J, Buerkler J, Guerini D, Brunner J, Carafoli E
出版信息
Biochemistry. 1984 Jan 31;23(3):400-3. doi: 10.1021/bi00298a002.
PMID:6704378
Abstract
3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine [( 125I]TID), a highly hydrophobic, carbene-generating photoreactive probe, labels calmodulin and some of its proteolytic fragments in the Ca2+-bound conformation only. It is assumed that [125I]TID labels hydrophobic sites exposed by the binding of Ca2+. The finding offers a new and powerful means to characterize calmodulin sites that play a role in the interaction with targets.
摘要
3-（三氟甲基）-3-（间-[¹²⁵I]碘苯基）重氮甲烷[（¹²⁵I]TID）是一种高度疏水的、能产生卡宾的光反应性探针，仅在Ca²⁺结合构象下标记钙调蛋白及其一些蛋白水解片段。据推测，[¹²⁵I]TID标记由Ca²⁺结合而暴露的疏水位点。这一发现为表征在与靶标相互作用中起作用的钙调蛋白位点提供了一种新的有力手段。
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