Chubb C, Chasalow F I
Endocrinology. 1984 Apr;114(4):1070-3. doi: 10.1210/endo-114-4-1070.
The malate shuttle has been hypothesized as a source of the intramitochondrial NADPH required for cholesterol side-chain cleavage. In the present report, the role of the malate shuttle in testicular steroidogenesis was investigated by employing mouse mutants deficient in cytoplasmic malic enzyme, a required component of the shuttle. Androgen-dependent parameters plus testosterone secretion by in vitro perfused testes were similar for the mutants and their normal siblings. The data suggest that an active malate shuttle is not required for cholesterol side-chain cleavage in mouse testes.
苹果酸穿梭机制被认为是胆固醇侧链裂解所需的线粒体内NADPH的来源。在本报告中，通过使用缺乏细胞质苹果酸酶（该穿梭机制的一个必需成分）的小鼠突变体，研究了苹果酸穿梭机制在睾丸类固醇生成中的作用。突变体及其正常同胞的雄激素依赖性参数以及体外灌注睾丸的睾酮分泌情况相似。数据表明，小鼠睾丸中胆固醇侧链裂解不需要活跃的苹果酸穿梭机制。
