Koerner K, Stampe D
Infusionsther Klin Ernahr. 1984 Feb;11(1):46-50.
Fresh frozen plasma (FFP), was shock-frozen to -25 degrees C within six hours after blood donation. The platelet count was reduced to 20 000/mm3. Aliquots were stored at -20 degrees C and -40 degrees C up to 24 months. Quick, PTT, factor V, VIII, IX, thrombin, antithrombin III, plasminogen, plasma-prekallikrein and kallikrein were determined monthly. With respect to the parameters investigated there was no significant difference between storage at -20 degrees C and -40 degrees C. Factor VIII loss was 10% after 12 months of storage. The activity of factor IX and V remained unchanged during 12 months, then factor V increased during storage. The other parameters did not change. Our study indicates quality of FFP seems not primarily depend on storage temperature, but an optimal preparation technique is much more important.
新鲜冰冻血浆（FFP）在献血后6小时内被速冻至-25摄氏度。血小板计数降至20000/mm³。等分试样在-20摄氏度和-40摄氏度下储存长达24个月。每月测定活化部分凝血活酶时间（Quick）、部分凝血活酶时间（PTT）、因子V、VIII、IX、凝血酶、抗凝血酶III、纤溶酶原、血浆前激肽释放酶和激肽释放酶。在所研究的参数方面，-20摄氏度和-40摄氏度储存之间没有显著差异。储存12个月后，因子VIII损失10%。因子IX和V的活性在12个月内保持不变，然后在储存期间因子V增加。其他参数没有变化。我们的研究表明，FFP的质量似乎并不主要取决于储存温度，而是最佳的制备技术更为重要。
