Lavie E, Shenberg C, Bitton M, Mechlis S
Int J Appl Radiat Isot. 1984 Feb;35(2):99-102. doi: 10.1016/0020-708x(84)90191-1.
The preparation of [99mTc]fibrinogen is described. The following factors which could affect the quality of this preparation were studied: pH, SnCl2 and protein concentrations, pre- and post-labeling incubation times and labeling temperature. A 90% labeling yield was achieved by incubating fibrinogen with SnCl2 at pH 9.8 for 22 h before adding the TcO-4 solution. The product obtained was stable and was 85% clottable. The x-ray fluorescence technique was employed to measure the amount of bound tin at different times.
描述了[99mTc]纤维蛋白原的制备方法。研究了以下可能影响该制备质量的因素：pH值、氯化亚锡和蛋白质浓度、标记前后的孵育时间以及标记温度。在加入高锝酸盐溶液之前，将纤维蛋白原与氯化亚锡在pH 9.8下孵育22小时，可实现90%的标记产率。得到的产物稳定，可凝性为85%。采用X射线荧光技术测量不同时间结合锡的量。
