Kinasewitz G T, Groome L J, Marshall R P, Diana J N
J Appl Physiol Respir Environ Exerc Physiol. 1984 Feb;56(2):355-63. doi: 10.1152/jappl.1984.56.2.355.
If the pulmonary lymphatics and/or interstitium of the lung participate in visceral pleural (VP) fluid exchange, then increasing the water content of the interstitial compartment by lymphatic ligation (L) should increase the filtration and/or decrease the absorption of fluid across the VP membrane. To test this hypothesis, the hydraulic conductivity (Lp) and diffusional permeability for protein (Pd) of the visceral pleura were determined in eight spontaneously breathing anesthetized chronic L dogs and seven sham-operated (S) controls. The left lung was enclosed in a water-impermeable membrane, creating a VP space; fluid flux was determined as the filtration or reabsorption of water and protein in the VP space. Intravascular and pleural pressures and the protein concentration of plasma and pleural fluid were monitored during two or more experimental periods in which different mixtures of plasma and saline were introduced in the VP space. At similar pulmonary capillary pressures, the extravascular lung water of the L animals [5.36 (+/- 0.32) g/g dry blood-free lung] was significantly (P less than 0.001) greater than that of the S dogs [3.79 (+/- 0.13)]. The Lp of the L dogs [6.8 (+/- 1.6) X 10(-7) ml X s-1 X cm-2 X Torr-1] was virtually identical (P greater than 0.8) to the 6.7 (+/- 1.4) ml X s-1 X cm-2 X Torr-1 of the S controls. Similarly, Pd in the L group [4.3 (+/- 0.5) X 10(-6) cm/s] was similar (P greater than 0.5) to that of the S animals [5.1 (+/- 1.1) X 10(-6) cm/s]. We conclude that neither the pulmonary lymphatics nor the interstitium of the lung contribute to VP fluid exchange.
如果肺淋巴管和/或肺间质参与脏层胸膜（VP）的液体交换，那么通过结扎淋巴管（L）增加间质腔的含水量应会增加液体通过VP膜的滤过和/或减少其吸收。为验证这一假设，在八只自主呼吸的麻醉慢性L组犬和七只假手术（S）对照组犬中测定了脏层胸膜的水力传导率（Lp）和蛋白质扩散通透性（Pd）。左肺被包裹在不透水的膜中，形成一个VP腔；液体通量被确定为VP腔内水和蛋白质的滤过或重吸收。在两个或更多实验期间监测血管内和胸膜压力以及血浆和胸液的蛋白质浓度，在此期间向VP腔内注入不同比例的血浆和盐水混合物。在相似的肺毛细血管压力下，L组动物的血管外肺水含量[5.36（±0.32）g/g无血干肺]显著高于（P<0.001）S组犬[3.79（±0.13）]。L组犬的Lp[6.8（±1.6）×10⁻⁷ml·s⁻¹·cm⁻²·Torr⁻¹]与S组对照的6.7（±1.4）ml·s⁻¹·cm⁻²·Torr⁻¹几乎相同（P>0.8）。同样，L组的Pd[4.3（±0.5）×10⁻⁶cm/s]与S组动物[5.1（±1.1）×10⁻⁶cm/s]相似（P>0.5）。我们得出结论，肺淋巴管和肺间质均不参与VP的液体交换。
