Maita T, Konno K, Ojima T, Matsuda G
J Biochem. 1984 Jan;95(1):167-77. doi: 10.1093/oxfordjournals.jbchem.a134581.
Amino acid sequences of the regulatory light chains of striated adductor muscle myosin from Ezo giant scallop (Patinopecten yessoensis) and akazara scallop (Chlamys nipponensis akazara) were determined. Tryptic peptides of each light chain were isolated and sequenced. The alignment of the tryptic peptides in each chain was deduced from the amino acid compositions and the partial sequences of peptic peptides of the Ezo giant scallop light chain. The light chains both consist of 156 residues. Heterogeneous residues, glutamic acid and aspartic acid were observed at the 155th position in the sequence of the akazara scallop light chain. A comparison of the Ezo giant scallop light chain with the glutamic acid-containing and aspartic acid-containing akazara light chains revealed 6 and 7 amino acid substitutions, respectively. When the presented sequences were compared with those of the regulatory light chains of gizzard, cardiac and skeletal muscle myosins, a strong homology was observed in the calcium binding region, but there were considerable heterogeneities in the N- and C-terminal regions.
测定了虾夷扇贝（Patinopecten yessoensis）和栉孔扇贝（Chlamys nipponensis akazara）横纹肌内收肌肌球蛋白调节轻链的氨基酸序列。分离并测定了每条轻链的胰蛋白酶肽段序列。根据虾夷扇贝轻链的氨基酸组成和胃蛋白酶肽段的部分序列推断出每条链中胰蛋白酶肽段的比对情况。两条轻链均由156个残基组成。在栉孔扇贝轻链序列的第155位观察到了谷氨酸和天冬氨酸这两种异质性残基。虾夷扇贝轻链与含谷氨酸和含天冬氨酸的栉孔扇贝轻链的比较分别揭示了6个和7个氨基酸替换。当将所呈现的序列与砂囊、心肌和骨骼肌肌球蛋白调节轻链的序列进行比较时，在钙结合区域观察到了很强的同源性，但在N端和C端区域存在相当大的异质性。
