Schreiber A B, Hoebeke J, Bergman Y, Haimovich J, Strosberg A D
J Immunol. 1978 Jul;121(1):19-23.
A new quantitative fluorometric binding assay that uses fluoresceinated aggregated IgG is proposed for the study of Fc receptors. The method was compared with a radiolabeling binding assay on three well characterized murine cell lines (38C-13, EL4, and BW). The apparent association constant of the binding and the amount of aggregated IgG bound per cell at saturation were calculated. The fluorometric assay enables the detection of 5 X 10(-10) M bound aggregated IgG. Inhibition studies with monomeric IgG, reduced and alkylated aggregated IgG, and aggregated F(ab')2 fragments of IgG confirmed the specificity of the assay. Staphylococcal protein A inhibited the binding of the aggregated IgG to Fc receptors.
提出了一种使用荧光素化聚集IgG的新型定量荧光结合测定法用于Fc受体的研究。该方法与放射性标记结合测定法在三种特性明确的小鼠细胞系（38C-13、EL4和BW）上进行了比较。计算了结合的表观缔合常数和饱和时每个细胞结合的聚集IgG量。荧光测定法能够检测到5×10⁻¹⁰ M结合的聚集IgG。用单体IgG、还原和烷基化的聚集IgG以及IgG的聚集F(ab')₂片段进行的抑制研究证实了该测定法的特异性。葡萄球菌蛋白A抑制聚集IgG与Fc受体的结合。
