Murray J M
J Cell Biol. 1984 Jan;98(1):283-95. doi: 10.1083/jcb.98.1.283.
The unicellular algae Distigma proteus contain a group of aligned microtubules associated with their cell membrane. The association is maintained in isolated membrane fragments. The membrane-microtubule complex also includes a crystalline array of membrane particles. The major peptide component of this array was identified by labeling whole cells with radioiodine. The entire complex of membrane, particles, and microtubules is sufficiently well ordered to permit reconstruction from electron micrographs by Fourier techniques. A three-dimensional model of the membrane array at a nominal resolution of 2.5 nm has been calculated. Some similarities were apparent between lattice spacings in the membrane array and in microtubules. Analysis of these lattice correlations suggests a way in which the array of membrane particles may serve as scaffolding for microtubule attachment.
单细胞藻类变形双鞭藻含有一组与其细胞膜相关联的排列整齐的微管。这种关联在分离的膜片段中得以维持。膜 - 微管复合体还包括膜颗粒的晶体阵列。通过用放射性碘标记整个细胞，鉴定出了该阵列的主要肽成分。膜、颗粒和微管的整个复合体排列得足够有序，以便通过傅里叶技术从电子显微照片进行重建。已经计算出了名义分辨率为2.5纳米的膜阵列三维模型。膜阵列和微管中的晶格间距之间存在一些明显的相似之处。对这些晶格相关性的分析表明了膜颗粒阵列可能作为微管附着支架的一种方式。
