膜-微管复合体的拆解与重组
Disassembly and reconstitution of a membrane-microtubule complex.
作者信息
Murray J M
出版信息
J Cell Biol. 1984 Apr;98(4):1481-7. doi: 10.1083/jcb.98.4.1481.
Abstract
The cell membrane of the unicellular algae Distigma proteus is associated with arrays of parallel microtubules. Fragments of the membrane-microtubule complex have been isolated and partially purified. The microtubules were stable in vitro at room temperature as well as at 0 degree C, but were specifically and rapidly disassembled by Ca2+. After removal of all endogenous microtubules, the membrane-microtubule complex could be reassembled from brain microtubule protein and denuded Distigma membrane fragments. The readded microtubules bound in a fixed orientation, and only to those regions of membrane that are normally associated with microtubules in vivo.
摘要
单细胞藻类变形双鞭藻的细胞膜与平行微管阵列相关联。膜 - 微管复合体的片段已被分离并部分纯化。微管在室温以及0℃下在体外是稳定的，但会被Ca2+特异性且快速地拆解。去除所有内源性微管后，膜 - 微管复合体可以由脑微管蛋白和去除微管的变形双鞭藻膜片段重新组装而成。重新添加的微管以固定方向结合，并且只结合到体内通常与微管相关联的那些膜区域。
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