Levy D E, Eveleigh J W
J Immunol Methods. 1978;22(1-2):131-42. doi: 10.1016/0022-1759(78)90065-0.
A method is presented for permanently converting an antigen immunosorbent into a purified antibody immunosorbent which retains specific reactivity for antigen. Following immunological reaction of the antibodies in an antiserum with the antigen adsorbent, the specific immunoglobulin is chemically bound to the antigen by means of the divalent crosslinking agent. Various such agents, including glutaraldehyde, suberimidate, a diazide, and isocyanates, were employed. The conditions of preparation using the first two were optimized, and the characteristics of the resulting immobilized antibodies were investigated. Applications in radioimmunoassay, preparative chromatography, and affinity-constant studies discussed in view of the unique attributes of these reagents.
本文介绍了一种将抗原免疫吸附剂永久性转化为对抗原保留特异性反应性的纯化抗体免疫吸附剂的方法。在抗血清中的抗体与抗原吸附剂发生免疫反应后，通过二价交联剂将特异性免疫球蛋白化学结合到抗原上。使用了各种此类试剂，包括戊二醛、亚胺酯、重氮化物和异氰酸酯。对使用前两种试剂的制备条件进行了优化，并研究了所得固定化抗体的特性。鉴于这些试剂的独特属性，讨论了它们在放射免疫测定、制备色谱和亲和常数研究中的应用。
