Sica G, Natoli C, Marchetti P, Piperno S, Iacobelli S
J Steroid Biochem. 1984 Jan;20(1):425-8. doi: 10.1016/0022-4731(84)90246-2.
It was previously shown that proliferation of CG-5 cells, an estrogen supersensitive variant of the MCF-7 cell line, is inhibited by tamoxifen starting three days after addition to culture medium. In this paper we report here on the effect of tamoxifen on CG-5 cell membrane morphology using scanning electron microscope. The addition of the antiestrogen to CG-5 cells causes marked alterations of cell surface, consisting of reduction in the length and the number of microvilli. Moreover, cells appear flattened and of reduced thickness. These modifications are more pronounced in cells plated at low density, but they are observed also in nearly confluent monolayers. In this case alterations are more evident in the center of cell islands, while at the periphery there is a certain mobility as reflected by the persistence of few microvilli and philopodia. These early effects of tamoxifen on cell membrane raise the possibility that parallel modifications in membrane function may occur which are responsible for alterations impairing transport mechanisms.
先前的研究表明，MCF-7细胞系的雌激素超敏变体CG-5细胞的增殖在加入培养基三天后开始受到他莫昔芬的抑制。在本文中，我们使用扫描电子显微镜报告了他莫昔芬对CG-5细胞膜形态的影响。向CG-5细胞中添加抗雌激素会导致细胞表面发生明显改变，包括微绒毛的长度和数量减少。此外，细胞显得扁平且厚度减小。这些改变在低密度接种的细胞中更为明显，但在几乎汇合的单层细胞中也能观察到。在这种情况下，细胞岛中心的改变更为明显，而在外围，由于少数微绒毛和丝状伪足的持续存在，存在一定的流动性。他莫昔芬对细胞膜的这些早期影响增加了膜功能可能发生平行改变的可能性，这些改变导致了损害转运机制的变化。
