Franklin R B, Khan M A, Costello L C
J Steroid Biochem. 1984 Mar;20(3):709-13. doi: 10.1016/0022-4731(84)90074-8.
Stimulation of mitochondrial aspartate aminotransferase (mAAT) activity by testosterone was determined in organ cultures of rat ventral prostate. The effect of testosterone on citrate accumulation in the culture medium was also determined. Testosterone stimulation of citrate accumulation and mAAT occurred in a dose dependent manner. Stimulation of mAAT activity occurred after a 1-3 h lag period and appeared to involve the synthesis of specific RNA since the response was inhibited by actinomycin D. Studies utilizing [3H]L-leucine indicated that unlike the total tissue, testosterone stimulated the incorporation of [3H]leucine into proteins of the mitochondrial fraction. The results suggested that mitochondrial proteins may be more sensitive to testosterone stimulation than cytosol proteins. The response was specific for mAAT since testosterone had no effect on mitochondrial malic dehydrogenase activity. The data suggested that testosterone may regulate prostate citrate content by the induction of mAAT in prostate mitochondria, which results in a source oxalacetic acid for citrate synthesis through transamination of aspartate by alpha ketoglutarate.
在大鼠腹侧前列腺器官培养物中测定了睾酮对线粒体天冬氨酸转氨酶（mAAT）活性的刺激作用。还测定了睾酮对培养基中柠檬酸盐积累的影响。睾酮对柠檬酸盐积累和mAAT的刺激呈剂量依赖性。在1-3小时的延迟期后出现了对mAAT活性的刺激，并且似乎涉及特定RNA的合成，因为放线菌素D抑制了该反应。利用[3H]L-亮氨酸的研究表明，与整个组织不同，睾酮刺激[3H]亮氨酸掺入线粒体部分的蛋白质中。结果表明，线粒体蛋白质可能比胞质溶胶蛋白质对睾酮刺激更敏感。由于睾酮对线粒体苹果酸脱氢酶活性没有影响，所以该反应对mAAT具有特异性。数据表明，睾酮可能通过诱导前列腺线粒体中的mAAT来调节前列腺柠檬酸盐含量，这通过α-酮戊二酸将天冬氨酸转氨作用为柠檬酸盐合成提供了草酰乙酸来源。
