Koroleva O N, Drutsa V L, Dolinnaia N G, Tsytovich A V, Shabarova Z A
Mol Biol (Mosk). 1984 Jan-Feb;18(1):146-60.
Two types of DNA-duplexes containing the repeating fragments of natural promoters have been obtained starting from synthetic oligodeoxyribonucleotides TGCATTATAA, AACTAGTT, AGTTAACT. Deca- and octanucleotides have been synthesized by solid phase method with stepwise or blockwise chain elongation. UV- and CD-spectroscopy has been used to study the physico-chemical properties of the synthetic oligonucleotides. Polycondensation of oligonucleotides induced by water-soluble carbodiimide (chemical ligation) or T4-polynucleotide ligase allowed to synthesize the promoter models. The degree of polymerization varied from 2 to 8 in case of chemical ligation and from 2 to 30 in case of enzymatic ligation. A new chain length regulation technique has been developed by means of addition of a terminator of polycondensation (unphosphorylated oligonucleotide) in the reaction mixture.
从合成的寡脱氧核糖核苷酸TGCATTATAA、AACTAGTT、AGTTAACT出发，已获得了两种含有天然启动子重复片段的DNA双链体。通过固相法逐步或逐段延长链，合成了十聚体和八聚体。利用紫外光谱和圆二色光谱研究了合成寡核苷酸的物理化学性质。通过水溶性碳二亚胺（化学连接）或T4多核苷酸连接酶诱导寡核苷酸缩聚，从而合成启动子模型。化学连接时聚合度从2到8不等，酶促连接时聚合度从2到30不等。通过在反应混合物中添加缩聚终止剂（未磷酸化的寡核苷酸），开发了一种新的链长调节技术。
