Morgans L F, Burns E R
Oncology. 1984;41(2):135-9. doi: 10.1159/000225808.
A single dose of 50 mg/kg of cyclophosphamide (cytoxan or CTX) was given to non-tumor-bearing mice and to mice bearing the Ehrlich ascites carcinoma (EAC). Circadian profiles in mitotic index and/or DNA-synthetic activity (DNA-SA: incorporation of tritiated thymidine into chemically isolated DNA) were monitored in normal organs and in the EAC. Mice were standardized to and kept on a 12 h: 12 h light-dark cycle with light from 06.00 to 18.00 h (CST or central standard time). In non-tumor-bearing mice, CTX at 05.00 h was more disruptive of the normal circadian profiles than was CTX at 17.00 h. CTX at 17.00 h was selected as the treatment to attempt to induce changes in DNA-SA of the EAC but concomitantly preserve the normal phasing of the circadian rhythms in the normal organs. Except for the bone marrow, CTX at 17.00 h did not perturb the normal circadian patterns in the normal organs (cornea, tongue, spleen, liver, ileum, rectum) of the EAC-bearing mice. CTX did cause a significant and prolonged inhibition of DNA-SA in the EAC. However, CTX-treated, EAC-bearing mice did not live significantly longer than saline-treated EAC-bearing mice.
给无肿瘤小鼠和携带艾氏腹水癌（EAC）的小鼠单次注射50mg/kg环磷酰胺（环磷酰铵或CTX）。监测正常器官和EAC中丝分裂指数和/或DNA合成活性[DNA-SA：将氚标记的胸腺嘧啶核苷掺入化学分离的DNA中]的昼夜变化情况。将小鼠标准化并维持在12小时：12小时的明暗循环中，光照时间为06:00至18:00（中部标准时间或CST）。在无肿瘤小鼠中，05:00给予CTX比17:00给予CTX对正常昼夜变化的干扰更大。选择17:00给予CTX进行治疗，试图诱导EAC的DNA-SA发生变化，但同时保持正常器官昼夜节律的正常相位。除骨髓外，17:00给予CTX并未扰乱携带EAC小鼠正常器官（角膜、舌、脾、肝、回肠、直肠）的正常昼夜模式。CTX确实导致EAC中DNA-SA受到显著且持久的抑制。然而，经CTX治疗的携带EAC的小鼠并不比经盐水治疗的携带EAC的小鼠存活时间显著延长。
