Thomas C M, Dyke K G
J Gen Microbiol. 1978 Jun;106(2):361-8. doi: 10.1099/00221287-106-2-361.
The properties of a temperature-sensitive mutant (ts39) of Staphylococcus aureus NCTC 8235 are described. After transfer to the restrictive temperature (42 degrees C), absorbance increased 10-to 20-fold but DNA content did not increase beyond 150 to 200% and cell division continued at a greatly reduced rate. On transfer back to the permissive temperature, both cell division and DNA synthesis resumed if the transfer occurred after less than 120 min at 42 degrees C. Resumption of DNA replication was blocked by chloramphenicol (100 microgram ml-1). The results are discussed with reference to possible defects in DNA replication. Replication of the plasmids pI258 and pT10501 and the chromosome were affected to a similar extent in ts39. Growth at 42 degrees C resulted in the appearance of an increased amount of pI258 DNA in a form that sedimented slowly in a sucrose gradient.
描述了金黄色葡萄球菌NCTC 8235的温度敏感突变体（ts39）的特性。转移到限制温度（42摄氏度）后，吸光度增加了10到20倍，但DNA含量增加不超过150%至200%，细胞分裂以大大降低的速率继续进行。如果在42摄氏度下少于120分钟后转移回允许温度，细胞分裂和DNA合成都会恢复。氯霉素（100微克/毫升）可阻止DNA复制的恢复。结合DNA复制中可能存在的缺陷对结果进行了讨论。在ts39中，质粒pI258和pT10501以及染色体的复制受到的影响程度相似。在42摄氏度下生长导致蔗糖梯度中沉降缓慢的pI258 DNA量增加。
