用于放射免疫测定的重组鸡生长激素。

Recombinant-derived chicken growth hormone used for radioimmunoassay.

作者信息

Proudman J A

出版信息

Proc Soc Exp Biol Med. 1984 Apr;175(4):417-9. doi: 10.3181/00379727-175-4-rc2.

Abstract

The use of recombinant-derived chicken growth hormone (rcGH) in an avian growth hormone (GH) radioimmunoassay (RIA) procedure is described. Antiserum to turkey GH bound 125I-labeled rcGH, and unlabeled rcGH or turkey GH displaced binding in a dose-related manner. The dose-response curves of sera and pituitary extract from chickens and turkeys were parallel to the rcGH standard curve. Sera from hypophysectomized (hypox) chickens and turkeys produced no dose-response and did not inhibit binding of labeled rcGH. Recovery of rcGH added to hypox sera was quantitative. Modification of the homologous turkey GH RIA protocol of Proudman and Wentworth (1) to use rcGH made possible either an increase in assay sensitivity or a 3-day reduction in incubation time.

摘要

本文描述了重组衍生鸡生长激素（rcGH）在禽生长激素（GH）放射免疫分析（RIA）程序中的应用。抗火鸡GH血清能结合125I标记的rcGH，未标记的rcGH或火鸡GH以剂量相关方式取代结合。鸡和火鸡血清及垂体提取物的剂量反应曲线与rcGH标准曲线平行。垂体切除（hypox）的鸡和火鸡的血清未产生剂量反应，也不抑制标记rcGH的结合。添加到hypox血清中的rcGH回收率是定量的。对Proudman和Wentworth（1）的同源火鸡GH RIA方案进行修改以使用rcGH，使得分析灵敏度得以提高或孵育时间缩短3天成为可能。