马催乳素同源放射免疫分析方法的建立与特性研究

Development and characterization of a homologous radioimmunoassay for equine prolactin.

作者信息

Roser J F, Chang Y S, Papkoff H, Li C H

出版信息

Proc Soc Exp Biol Med. 1984 Apr;175(4):510-7. doi: 10.3181/00379727-175-41829.

Abstract

A specific and sensitive homologous radioimmunoassay has been developed for equine prolactin, suitable for measuring prolactin concentrations in serum of horses. The sensitivity of the assay ranged from 0.4 to 0.6 ng/ml and the intra- and inter-assay coefficients of variation averaged 6.9 and 15.4%, respectively, for five doses of hormone. Cross-reactivity with other mammalian and nonmammalian prolactins and growth hormones was less than 20 and 0.3%, respectively. Cross-reactivity with equine growth hormone was less than 0.07%. Equine serum and pituitary extracts showed parallel dilution-response curves with equine prolactin. The percentage recovery of exogenous equine prolactin in serum was 89%. Preliminary analysis of several physiological samples (stallions, pregnant, and nonpregnant mares) yielded values from 0.6 to 12.0 ng/ml.

摘要

已开发出一种针对马催乳素的特异性和灵敏性均高的同源放射免疫测定法，适用于测定马血清中的催乳素浓度。该测定法的灵敏度范围为0.4至0.6纳克/毫升，对于五剂激素，测定内和测定间变异系数平均分别为6.9%和15.4%。与其他哺乳动物和非哺乳动物的催乳素及生长激素的交叉反应率分别小于20%和0.3%。与马生长激素的交叉反应率小于0.07%。马血清和垂体提取物与马催乳素呈现平行的稀释 - 反应曲线。血清中外源马催乳素的回收率为89%。对几个生理样本（种马、怀孕和未怀孕母马）的初步分析得出的值为0.6至12.0纳克/毫升。