[裂解液pH值对完整及辐照艾氏腹水瘤细胞核质沉降特性的影响]
[Effect of the pH of the lysing solution on the sedimentation properties of the nucleoid of intact and irradiated Ehrlich ascites tumor cells].
作者信息
Ingel' F I, Kutman A I, Riabchenko N I
出版信息
Radiobiologiia. 1984 Jan-Feb;24(1):73-6.
A study was made of the sedimentation properties of Ehrlich ascites tumor cell nucleotide obtained by cell lysis in 1.95 M NaCl, 0.1 M EDTA, 0.02 M tris, and 0.5% triton X-100, at pH 5.0 and 8.0. It was shown that the nucleotide obtained at pH 5.0 has a more compact structure than that obtained at pH 8.0. Irradiation of cells leads to relaxation of the nucleotide decreasing the rate of its sedimentation in a neutral sucrose gradient. The influence of irradiation on the sedimentation properties is more pronounced with pH 5.0 than 8.0.
对通过在pH值为5.0和8.0的条件下，于1.95M氯化钠、0.1M乙二胺四乙酸（EDTA）、0.02M三羟甲基氨基甲烷（tris）和0.5% Triton X - 100中进行细胞裂解而获得的艾氏腹水肿瘤细胞核苷酸的沉降特性进行了研究。结果表明，在pH 5.0时获得的核苷酸比在pH 8.0时获得的具有更紧密的结构。细胞照射导致核苷酸松弛，降低了其在中性蔗糖梯度中的沉降速率。与pH 8.0相比，在pH 5.0时照射对沉降特性的影响更为明显。
Zotero 插件