Kluft C, Los P, Jie A F
Thromb Res. 1984 Feb 15;33(4):419-25. doi: 10.1016/0049-3848(84)90081-1.
Two molecular forms of alpha 2-antiplasmin (PB = plasminogen-binding; NPB = non-PB) exist. We have studied the extent of binding of these two forms to fibrin by factor XIII. A modified crossed immunoelectrophoresis technique which separately determines both forms showed that, compared with plasma, the PB-form is reduced by 31 +/- 11% (SD) in serum of twelve individuals and the NPB form by only 6%. Laurell assay showed a reduction of 18 +/- 9% (n = 12) in total alpha 2-antiplasmin antigen (PB + NPB) in serum; the immediate plasmin inhibition test (mainly recording the PB-form) revealed 35 +/- 6% (n = 12) reduction in inhibition. Coagulation of blood, platelet-rich and platelet-poor plasma yielded comparable results. No decrease in alpha 2-antiplasmin or change in its composition was observed when factor XIII-deficient plasma was clotted. Fibrin binding was not significantly different from normal in either plasminogen-depleted or plasminogen-enriched plasma. It is concluded that the PB-form of alpha 2-antiplasmin becomes selectively bound to fibrin.
存在两种分子形式的α2 - 抗纤溶酶（PB = 纤溶酶原结合型；NPB = 非PB型）。我们研究了这两种形式通过因子XIII与纤维蛋白的结合程度。一种能分别测定这两种形式的改良交叉免疫电泳技术表明，与血浆相比，在12名个体的血清中，PB型降低了31±11%（标准差），而NPB型仅降低了6%。 Laurell分析法显示血清中总α2 - 抗纤溶酶抗原（PB + NPB）降低了18±9%（n = 12）；即时纤溶抑制试验（主要记录PB型）显示抑制作用降低了35±6%（n = 12）。血液、富血小板血浆和贫血小板血浆的凝固产生了类似的结果。当因子XIII缺乏的血浆凝固时，未观察到α2 - 抗纤溶酶减少或其组成发生变化。在纤溶酶原耗尽或纤溶酶原富集的血浆中，纤维蛋白结合与正常情况无显著差异。结论是α2 - 抗纤溶酶的PB型选择性地与纤维蛋白结合。
