Raghavan V
J Cell Biol. 1977 May;73(2):521-6. doi: 10.1083/jcb.73.2.521.
Continued DNA synthesis in the generative cell nucleus, followed by mitosis and cytokinesis, results in the formation of pollen embryoids in cultured anthers of H. niger. In contrast, the nucleus of the vegetative cell undergoes no DNA synthesis after it is cut off, or synthesizes DNA only during a limited number of cell cycles. DNA synthetic patterns in the generative and vegetative cell nuclei confirm the ontogeny of embryoids described in this plant.
在黑种草培养的花药中，生殖细胞核中持续的DNA合成，随后进行有丝分裂和胞质分裂，导致花粉胚状体的形成。相比之下，营养细胞的细胞核在与生殖细胞分离后不进行DNA合成，或者仅在有限数量的细胞周期中合成DNA。生殖细胞核和营养细胞核中的DNA合成模式证实了该植物中描述的胚状体的个体发育。
