Studies on the pathogenicity of spiroplasmas for Drosophila pseudoobscura.

Representatives of several currently available spiroplasma serovars were used in feeding and injection experiments involving Drosophila pseudoobscura adult flies in order to evaluate the host-range of the spiroplasmas and to determine their pathogenicity for flies. Many of the isolates injected into flies either do not survive or grow poorly, and have no negative effects on longevity or fecundity. Two spiroplasma strains, honey-bee (serovar I-2) and cornstunt (serovar I-3), can grow to high titres in injected flies but are not pathogenic. In addition to the special pathology of male lethality caused by the sex-ratio organisms (spiroplasmas) which occur naturally in several neotropical species of Drosophila, two spiroplasma serovars resulted in the death of injected flies. Spiroplasmas isolated from syrphid flies (serovar VIII) and from Cotinus beetles (serovar IX) induced the appearance of symptoms of pathology that eventually culminated in death. All such flies which were examined for the presence of spiroplasmas in their haemolymph showed them to be present in very high concentrations. The numbers of progeny which these flies produced were greatly reduced, but did consist of equal numbers of flies of both sexes. Spiroplasmas from their infected female parents were not vertically (transovarially) transmitted. The results of the feeding experiments were all negative: none of the spiroplasmas persisted in the gut or appeared in the haemolymph. None of the flies which had been fed the spiroplasma suspension displayed any pathology.