Pathogenicity of mollicutes for insects: possible use in biological control.

Acholeplasmas, spiroplasmas and other non-helical sterol-requiring mycoplasmas of unknown phylogenetic affinity inhabit insects. Of these, only spiroplasmas are known to be pathogenic. Group I-2 spiroplasmas, or Spiroplasma apis, especially in combination with other organisms, reduce honey-bee longevity. Plant pathogenic mycoplasma-like organisms are often found intracellularly in insects. Spiroplasmas are found predominantly in the gut lumen or haemolymph (or both) of their insect hosts. Pathogenicity of mycoplasmas is usually altered by extended passage in unusual hosts, in only one of two alternate hosts, or in culture media. Enhancement of experimental pathogenicity may occur with extended cultural passages, but maintenance of natural pathogenicity must be accomplished by continuous exposure to the usual host. Recent data provide new information on the ecology of pathogenicity. Spiroplasmas from unique habitats also tend to be unique. Spiroplasmas isolated from flowers appear to be adapted to insect species that frequent floral surfaces. Group IV spiroplasmas have been isolated from members of 4 holometabolous insect orders (including Lepidoptera), all of which visit flowers. Social or predatory insects, or insects with an "aggregation" phase in their life histories, also appear to be prone to spiroplasma infection. Some insect species which harbor spiroplasmas also carry infections of other mollicutes, some of which involve the haemolymph. Appearance of spiroplasmas in adult insects in nature is strongly affected by seasonality. Extensive tests of the host ranges of the new insect mollicutes will be required before their suitability for biological control can be evaluated.