Simpson R U, Wichmann J K, Paaren H E, Schnoes H K, DeLuca H F
Arch Biochem Biophys. 1984 Apr;230(1):21-9. doi: 10.1016/0003-9861(84)90082-1.
A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites.
已成功培养出大鼠肾脏上皮细胞的原代融合培养物。这些细胞具有一种对1,25 - 二羟基维生素D3具有3.2 - 3.4 S高亲和力、低容量的结合蛋白。它们将25 - 羟基维生素D3代谢为至少五种代谢产物。其中两种已被鉴定为1,25 - 二羟基维生素D3和24,25 - 二羟基维生素D3。另外两种已通过物理数据和共色谱法鉴定为反式19 - 去甲 - 10 - 氧代 - 25 - 羟基维生素D3，另一种为其顺式异构体。这两种“代谢产物”在体内未被观察到，但其中一种（顺式）在正相高效液相色谱上与1,25 - 二羟基维生素D3共迁移。因此，仅在高效液相色谱上进行共色谱分析不足以鉴定关键的维生素D代谢产物。
