人粒细胞中D-α-生育酚对单线态氧的猝灭作用。

Quenching of singlet oxygen by D-alpha-tocopherol in human granulocytes.

作者信息

Littarru G P, Lippa S, De Sole P, Oradei A, Dalla Torre F, Macrì M

出版信息

Biochem Biophys Res Commun. 1984 Mar 30;119(3):1056-61. doi: 10.1016/0006-291x(84)90881-7.

Abstract

The ability of D-alpha-tocopherol to act as a quencher of (1)0(2) (singlet oxygen) was tested with a biological source of (1)0(2), namely the phagocytosis activated myeloperoxidase contained in the homogenate of human circulating polymorphonuclear leukocytes. With this system, the (1)0(2) quenching efficiency of exogenously added D-alpha-tocopherol was estimated from its inhibitory effect on the luminol amplified chemiluminescence. This inhibitory effect was dose dependent. D-alpha-tocopherol was also efficient in quenching the chemiluminescence generated through the H2O2-horseradish system. In both systems the quenching effect may be almost entirely "physical", since very little tocopherol was destroyed when compared to the relatively large amount of H2O2 consumed.

摘要

用一种生物源单线态氧（¹O₂），即人循环多形核白细胞匀浆中吞噬作用激活的髓过氧化物酶，测试了D-α-生育酚作为¹O₂猝灭剂的能力。在这个系统中，根据外源性添加的D-α-生育酚对鲁米诺增强化学发光的抑制作用，估算其¹O₂猝灭效率。这种抑制作用呈剂量依赖性。D-α-生育酚在猝灭通过H₂O₂-辣根过氧化物酶系统产生的化学发光方面也很有效。在这两个系统中，猝灭作用几乎完全可能是“物理性的”，因为与消耗的相对大量的H₂O₂相比，只有极少的生育酚被破坏。