Mazzuolo S, Marotta M, Fiorillo P, Tajana G
Boll Soc Ital Biol Sper. 1984 Feb 28;60(2):265-71.
The fine structure of five lines of medulloblastoma cells in long-term cell cultures are described. The cell cultures were fixed in situ by the rapid exchange of the medium for the fixative solution consisting of 3% glutaraldhyde in 0.1 M Na cacodylate-HC1 buffer with 0.1 M sucrose (pH 7,2, total osmolality 510 mOsm, vehicle osmolality 300 mOsm). The cultures were subsequently postfixed in 1% OsO4 in water at room temperature and dehydrated in a graded series of ethanol solutions. In the second change of 100% ethanol they were stained with 2% uranyl acetate for 10 minutes. They described the structural characteristics of these cells in relation to the various phases of the culture, is similar to the well known of in vitro cellular embryonal systems (spongioblasts).
描述了长期细胞培养中五株髓母细胞瘤细胞的精细结构。通过将培养基快速换为固定液来原位固定细胞培养物，固定液由0.1 M 二甲胂酸钠-盐酸缓冲液（含0.1 M蔗糖，pH 7.2，总渗透压510 mOsm，载体渗透压300 mOsm）中的3%戊二醛组成。随后将培养物在室温下用1%四氧化锇进行后固定，并在一系列梯度乙醇溶液中脱水。在第二次更换100%乙醇时，用2%醋酸铀染色10分钟。他们描述了这些细胞在培养不同阶段的结构特征，类似于体外细胞胚胎系统（成胶质细胞）中众所周知的特征。
