Abbey M, Calvert G D, Barter P J
Biochim Biophys Acta. 1984 May 11;793(3):471-80. doi: 10.1016/0005-2760(84)90265-0.
A lipid transfer protein has been purified from the lipoprotein-free fraction of rabbit plasma. Rabbit lipid transfer protein, which was purified 600-700-fold with a 4% recovery, has an apparent Mr of 68 000 and facilitates the transfer of isotopically labelled cholesteryl ester, triacylglycerol and phosphatidylcholine between low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Rabbit lipid transfer protein, which appears to be very similar to the cholesteryl ester exchange protein previously purified from human plasma, was incubated with pig plasma at 37 degrees C for up to 6 h. Analysis of very-low-density lipoprotein (VLDL, d less than 1.006 g/ml), LDL (d 1.019-1.063 g/ml) and HDL (d 1.090-1.21 g/ml) after incubation showed that lipid transfer protein had a marked effect on the composition of the lipoprotein classes. The VLDL became enriched with cholesteryl ester and depleted of triacylglycerol. The LDL and HDL became enriched with triacylglycerol. In addition to these changes in lipid composition there were also changes in apolipoprotein composition. The most prominent change in apolipoprotein distribution was a marked increase in the apolipoprotein E content of LDL which was observed only after incubation in the presence of lipid transfer protein.
一种脂质转运蛋白已从兔血浆的无脂蛋白组分中纯化出来。兔脂质转运蛋白经纯化后回收率为4%，纯化倍数为600 - 700倍，其表观分子量为68000，可促进低密度脂蛋白（LDL）和高密度脂蛋白（HDL）之间同位素标记的胆固醇酯、三酰甘油和磷脂酰胆碱的转运。兔脂质转运蛋白似乎与人血浆中先前纯化的胆固醇酯交换蛋白非常相似，将其与猪血浆在37℃孵育长达6小时。孵育后对极低密度脂蛋白（VLDL，d小于1.006 g/ml）、低密度脂蛋白（LDL，d 1.019 - 1.063 g/ml）和高密度脂蛋白（HDL，d 1.090 - 1.21 g/ml）进行分析表明，脂质转运蛋白对脂蛋白类别的组成有显著影响。VLDL富含胆固醇酯，三酰甘油减少。LDL和HDL富含三酰甘油。除了脂质组成的这些变化外，载脂蛋白组成也有变化。载脂蛋白分布最显著的变化是仅在脂质转运蛋白存在下孵育后观察到的LDL中载脂蛋白E含量的显著增加。
