Caprasse M, Houssier C
Biochimie. 1984 Jan;66(1):31-41. doi: 10.1016/0300-9084(84)90189-5.
The mode of binding of 5,6-dihydroflavopereirine and sempervirine to DNA has been investigated by absorption spectrophotometry, circular and electric linear dichroism, fluorescence and fluorescence polarization, viscosity increase of sonicated linear DNA and circular DNA unwinding. Although the spectroscopic properties of both compounds bound to DNA resembled those reported in our previous study of DNA complexes with two other alkaloids, and observed with planar intercalating compounds, only sempervirine was able to unwind circular DNA. The latter drug however showed signs characteristic of aggregation at the surface of the polyion. The differences between the behaviours of the four alkaloids so far investigated by us are interpreted on the basis of different extent of penetration of the chromophore ring into the DNA helix.
通过吸收分光光度法、圆二色和电线性二色性、荧光和荧光偏振、超声线性DNA的粘度增加以及环状DNA解旋，研究了5,6-二氢黄酮哌啶和万年青碱与DNA的结合模式。尽管这两种与DNA结合的化合物的光谱性质与我们之前对DNA与其他两种生物碱复合物的研究中报道的性质相似，并且在平面嵌入化合物中也观察到了这种性质，但只有万年青碱能够解开环状DNA。然而，后一种药物在聚离子表面表现出聚集的特征迹象。我们目前研究的四种生物碱行为之间的差异是根据发色团环渗透到DNA螺旋中的不同程度来解释的。
