Speers W C, Altmann M
Cancer Res. 1984 May;44(5):2136-43.
Murine embryonal carcinoma tumors were induced to differentiate in vivo using retinoic acid. Six mice bearing seven tumors survived more than 100 days after treatment. Histological samples of these tumors showed no residual embryonal carcinoma cells, and, for the most part, they were benign cystic teratomas. Three tumors, in addition to the benign tissue, had solid, mitotically active areas. Two of these tumors upon transplantation gave rise to progressively growing, potentially lethal tumors which have proven to be permanently transplantable cell lines. Using techniques of light and electron microscopy, immunohistochemistry, flow microfluorometry, and cytogenetics, we have characterized these lines. One is a chondrosarcoma, and one is a glioma:chondrosarcoma mixture. Both are chromosomally different from the parent embryonal carcinoma stem cell line, but both were clearly derived from it.
使用视黄酸在体内诱导小鼠胚胎癌肿瘤分化。六只携带七个肿瘤的小鼠在治疗后存活超过100天。这些肿瘤的组织学样本未显示残留的胚胎癌细胞，并且在很大程度上它们是良性囊性畸胎瘤。除了良性组织外，三个肿瘤还有实性、有丝分裂活跃的区域。其中两个肿瘤移植后产生了逐渐生长、可能致命的肿瘤，这些肿瘤已被证明是可永久移植的细胞系。我们使用光镜和电镜技术、免疫组织化学、流式细胞荧光测定法和细胞遗传学对这些细胞系进行了表征。一个是软骨肉瘤，一个是胶质瘤：软骨肉瘤混合物。两者在染色体上都与亲本胚胎癌干细胞系不同，但两者都明显源自该细胞系。
