Gibson R A, Lattanzio L, McGee H
Clin Chem. 1984 May;30(5):784-7.
Metronidazole and its known metabolites in plasma can be rapidly separated by a "high-pressure" liquid-chromatographic method that can also be adapted for rapid determination of tinidazole. Samples deproteinized with trichloroacetic acid (50 g/L final concentration) undergo isocratic separation on a reversed-phase C18 column eluted with an 8/92 (by vol) mixture of acetonitrile/KH2PO4 (5 mmol/L, pH 3.0). The method is sensitive, reliably detecting as little as 25 micrograms of metronidazole and (or) its metabolites per milliliter of plasma. The detector response varied linearly with concentration for all compounds tested over a wide range (25-500 micrograms/L). Within-day and between-day variation was generally less than 2.5% for all concentrations of all compounds tested. Various other antibiotics tested did not interfere.
甲硝唑及其在血浆中的已知代谢物可通过一种“高压”液相色谱法快速分离，该方法也可适用于替硝唑的快速测定。用三氯乙酸（终浓度50 g/L）进行脱蛋白处理的样品，在反相C18柱上进行等度分离，用乙腈/KH2PO4（5 mmol/L，pH 3.0）的8/92（体积比）混合物洗脱。该方法灵敏，每毫升血浆中能可靠检测低至25微克的甲硝唑和（或）其代谢物。在所测试的宽浓度范围（25 - 500微克/升）内，所有化合物的检测器响应与浓度呈线性变化。对于所测试的所有化合物的所有浓度，日内和日间变化通常小于2.5%。所测试的其他各种抗生素均无干扰。
