Nakashima T, Inoki M, Nakanishi Y
Eur J Drug Metab Pharmacokinet. 1984 Jan-Mar;9(1):73-8. doi: 10.1007/BF03189607.
Nifedipine (10 mg) was administered orally to 8 normotensive volunteers. Various measurements such as concentration in serum, blood pressure, and heart rate, were made at 0, 20', 40', 1, 2, 3, 4, 6, 8 and 24 h after administration. The sample serum, internal standard (diethyl 2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylate), HCl and NaNO2 were allowed to react and GLC-ECD was carried out after extration with organic solvent. The retention time was 3 min for nifedipine and 4 min for internal standard. Absorption curves were classified into 3 types: a rapid absorption rate (2 subjects) in which the maximum measured nifedipine concentration (70-80 ng/ml) was obtained, within 20-40 min after administration, a slower absorption rate (3 subjects) and an intermediate absorption rate (3 subjects) and an intermediate absorption rate (3 subjects). A decrease in systolic blood pressure was observed, but no significant difference in diastolic blood pressure and heart rate could be noted among the volunteers.
给8名血压正常的志愿者口服硝苯地平（10毫克）。在给药后0、20分钟、40分钟、1小时、2小时、3小时、4小时、6小时、8小时和24小时进行了各种测量，如血清浓度、血压和心率。使样本血清、内标（2,6-二甲基-4-(2-硝基苯基)-3,5-吡啶二羧酸二乙酯）、盐酸和亚硝酸钠反应，并在用有机溶剂萃取后进行气相色谱-电子捕获检测。硝苯地平的保留时间为3分钟，内标的保留时间为4分钟。吸收曲线分为3种类型：快速吸收型（2名受试者），给药后20 - 40分钟内测得硝苯地平最大浓度（70 - 80纳克/毫升）；吸收较慢型（3名受试者）；吸收中间型（3名受试者）。观察到收缩压下降，但志愿者之间舒张压和心率无显著差异。
