Chemnitius J M, Zech R
Int J Biochem. 1984;16(4):361-7. doi: 10.1016/0020-711x(84)90133-2.
A standard procedure for lipid-extraction of lyophilized hen brain material is described. Nine carboxylesterase isoenzymes (EC 3.1.1.1) are identified in lipid-extracted lyophilized material (LELM) using kinetic analysis of organophosphate inhibition. Total phenyl valerate (PV) hydrolysing carboxylesterase activity in LELM is 43.3 U X g-1. Two carboxylesterase isoenzymes of LELM are classified as neurotoxic esterases (NTEA and NTEB). Using n-octylglucoside 51% of the water-insoluble neurotoxic esterase activity from LELM are solubilized. Six carboxylesterase isoenzymes including NTEA (6.5 U X 1(-1] and NTEB (4.2 U X 1(-1] are present in the solubilized preparation. Throughout purification and separation steps carboxylesterase isoenzymes are identified by their rate constants for the reaction with organophosphorus inhibitors.
本文描述了一种用于冻干鸡脑材料脂质提取的标准程序。通过对有机磷抑制的动力学分析，在脂质提取的冻干材料（LELM）中鉴定出9种羧酸酯酶同工酶（EC 3.1.1.1）。LELM中总苯基戊酸（PV）水解羧酸酯酶活性为43.3 U X g-1。LELM的两种羧酸酯酶同工酶被归类为神经毒性酯酶（NTEA和NTEB）。使用正辛基葡萄糖苷可溶解LELM中51%的水不溶性神经毒性酯酶活性。溶解制剂中存在六种羧酸酯酶同工酶，包括NTEA（6.5 U X 1(-1]）和NTEB（4.2 U X 1(-1]）。在整个纯化和分离步骤中，通过羧酸酯酶同工酶与有机磷抑制剂反应的速率常数来鉴定它们。
