Merville M P, Decuyper J, Piette J, Calberg-Bacq C M, Van de Vorst A
Invest Ophthalmol Vis Sci. 1984 May;25(5):573-80.
Promazine derivatives induce cross-linking of bovine lens crystallins in vitro by irradiation with near-ultraviolet (UV) light in the presence of O2, as revealed by electrophoresis after denaturation. With the five derivatives tested (promazine [PZ], chlorpromazine [CPZ], triflupromazine [ TFPZ ], methoxypromazine [ MTPZ ], and acepromazine [ ACPZ ] ), single-hit kinetics are observed. Evidence implicating the cation radicals of the PZ derivatives as the causative agent of this in vitro effect is presented. Hydroxyl radicals do not appear to be involved in the photo-cross-linking reaction. Sodium ascorbate protects against damage induced either by PZ derivatives plus light or by PZ cation radicals in the dark. These findings are discussed with respect to development of cataracts induced by these drugs in vivo.
如变性后电泳所示，在有氧存在的情况下，用近紫外线（UV）照射，异丙嗪衍生物可在体外诱导牛晶状体晶状体蛋白交联。在所测试的五种衍生物（异丙嗪[PZ]、氯丙嗪[CPZ]、三氟拉嗪[TFPZ]、甲氧异丙嗪[MTPZ]和乙酰丙嗪[ACPZ]）中，观察到单击动力学。本文提供了证据，表明PZ衍生物的阳离子自由基是这种体外效应的致病因子。羟基自由基似乎不参与光交联反应。抗坏血酸钠可防止由PZ衍生物加光或黑暗中的PZ阳离子自由基诱导的损伤。结合这些药物在体内诱发白内障的情况对这些发现进行了讨论。
