Obinata T, Saitoh O, Takano-Ohmuro H
J Biochem. 1984 Feb;95(2):585-8. doi: 10.1093/oxfordjournals.jbchem.a134643.
The effect of innervation on the transition of tropomyosin, troponin T, and myosin isozyme during chicken breast muscle development was examined by denervating the muscle at various ages after hatching. The types of proteins were characterized by 2-D electrophoresis for tropomyosin, immunoblotting for troponin T and pyro-phosphate acrylamide gel electrophoresis for myosin isozymes. As judged by the types of these three proteins, when neonatal muscle was denervated, the protein isoform transition from the neonatal to adult state was interrupted, whereas the denervation of mature muscle caused the reappearance of the neonatal forms of proteins. The present results indicate that differentiation from the neonatal state to the adult state and the maintenance of the adult state are controlled by some factors related to nerves.
通过在孵化后的不同年龄对鸡胸肌进行去神经支配，研究了神经支配对鸡胸肌发育过程中肌钙蛋白、肌钙蛋白T和肌球蛋白同工酶转变的影响。通过二维电泳对肌钙蛋白进行蛋白质类型鉴定，通过免疫印迹法对肌钙蛋白T进行鉴定，通过焦磷酸丙烯酰胺凝胶电泳对肌球蛋白同工酶进行鉴定。根据这三种蛋白质的类型判断，当新生肌肉去神经支配时，蛋白质异构体从新生状态向成年状态的转变被中断，而成熟肌肉去神经支配则导致蛋白质新生形式的重新出现。目前的结果表明，从新生状态到成年状态的分化以及成年状态的维持受一些与神经相关的因素控制。
