Kanofsky J R
J Biol Chem. 1984 May 10;259(9):5596-600.
Singlet oxygen production in the chloroperoxidase-hydrogen peroxide-halide system was studied using 1268 nm chemiluminescence. With chloride or bromide ions, singlet oxygen is produced by the mechanism (formula; see text) (formula; see text) where X- is chloride or bromide ion. Under conditions where there is high enzyme activity and when Reaction B is fast relative to Reaction A, singlet oxygen is produced in near stoichiometric amounts. In contrast, when Reaction A is fast relative to Reaction B, oxidized halogen species (chlorine and hypochlorous acid for chloride ion; bromide, tribromide ion, and hypobromous acid for bromide ion) are the principle reaction products. With iodide ion, no 1268 nm chemiluminescence was detected. Past studies have shown that iodine and iodate ion are the major end products of this system.
利用1268纳米化学发光研究了氯过氧化物酶 - 过氧化氢 - 卤化物体系中单线态氧的产生。对于氯离子或溴离子，单线态氧通过以下机制产生（公式；见原文）（公式；见原文），其中X⁻为氯离子或溴离子。在酶活性高且反应B相对于反应A快速的条件下，单线态氧以接近化学计量的量产生。相比之下，当反应A相对于反应B快速时，氧化态卤素物种（氯离子对应的氯和次氯酸；溴离子对应的溴、三溴离子和次溴酸）是主要反应产物。对于碘离子，未检测到1268纳米的化学发光。过去的研究表明，碘和碘酸根离子是该体系的主要最终产物。
