Burdman J A, Calabrese M T, Romano M I, Carricarte V C, MacLeod R M
J Endocrinol. 1984 May;101(2):197-201. doi: 10.1677/joe.0.1010197.
In the anterior pituitary gland changes in prolactin synthesis and in the incorporation of [3H]thymidine into DNA are coincident under several experimental conditions. We investigated whether these changes are obligatory, thus indicating a regulatory mechanism common to the synthesis of both macromolecules. Alternatively, the parallel changes may represent similar responses to various stimuli operating through different pathways. The administration of alpha-methyl-p-tyrosine (alpha MpT) to rats stimulated the incorporation of [3H]leucine into prolactin and [3H]thymidine into DNA. When the effectiveness of oestrogen was suppressed by ovariectomy or by blockage of oestrogen receptors by the antioestrogen clomiphene, alpha MpT stimulated the synthesis of prolactin but not the incorporation of [3H]thymidine into pituitary DNA. The results clearly indicate two independent mechanisms regulating the synthesis of prolactin and DNA in the anterior pituitary gland.
在几种实验条件下，垂体前叶催乳素合成的变化以及[3H]胸腺嘧啶核苷掺入DNA的变化是同时发生的。我们研究了这些变化是否是必然的，从而表明这两种大分子合成存在共同的调节机制。或者，平行变化可能代表对通过不同途径起作用的各种刺激的类似反应。给大鼠注射α-甲基-p-酪氨酸（αMpT）可刺激[3H]亮氨酸掺入催乳素以及[3H]胸腺嘧啶核苷掺入DNA。当通过卵巢切除术或抗雌激素克罗米酚阻断雌激素受体来抑制雌激素的作用时，αMpT可刺激催乳素的合成，但不能刺激[3H]胸腺嘧啶核苷掺入垂体DNA。结果清楚地表明，存在两种独立的机制调节垂体前叶催乳素和DNA的合成。
