采用反相高效液相色谱-荧光检测法对吐根碱和吐根酚碱进行定量分析。
Quantitative analysis of emetine and cephaeline by reversed-phase high performance liquid chromatography with fluorescence detection.
作者信息
Crouch D J, Moran D M, Finkle B S, Peat M A
出版信息
J Anal Toxicol. 1984 Mar-Apr;8(2):63-5. doi: 10.1093/jat/8.2.63.
A versatile method for the quantitation of emetine and cephaeline in biological samples is described. Two milliliters of samples containing N-propylprocainamide as the internal standard are buffered to pH 9 and extracted with n-butyl chloride. After subsequent back extraction into 0.01 M hydrochloric acid, a portion of the acid layer is analyzed by reversed-phase high performance liquid chromatography with fluorescence detection. Routinely, the minimum level of detection for both drugs is 5 ng/mL and linearity is demonstrated from 5 to 2500 ng/mL.
本文描述了一种用于定量生物样品中土的宁和吐根酚碱的通用方法。将含有N - 丙基普鲁卡因酰胺作为内标的两毫升样品缓冲至pH 9，并用正丁基氯萃取。随后反萃取到0.01 M盐酸中后，取一部分酸层通过带荧光检测的反相高效液相色谱进行分析。通常，两种药物的最低检测限均为5 ng/mL，线性范围为5至2500 ng/mL。
Zotero 插件