用于放射性配体结合分析的单-125I-血管紧张素II的制备及一步纯化

Preparation and one-step purification of mono-125I-angiotensin II for radioligand binding assays.

作者信息

Speth R C, Husain A

出版信息

J Pharmacol Methods. 1984 Apr;11(2):137-50. doi: 10.1016/0160-5402(84)90023-8.

Abstract

A one-step purification of mono-125I-angiotensin II prepared by the chloramine T procedure is described. The purification is effected on a cellulose cation exchange column with isocratic elution by 50 mM sodium acetate, pH 5.0. The purity of the mono-125I-angiotensin II was determined by thin layer chromatography, high pressure liquid chromatography, enzymatic digestion, radioreceptor assay, and radioimmunoassay. Preparation and purification of mono-125I-angiotensin II by this procedure offers significant advantages over existing methods for its preparation in terms of purity, simplicity, efficiency, and cost.

摘要

本文描述了一种通过氯胺T法制备的单-125I-血管紧张素II的一步纯化方法。该纯化过程在纤维素阳离子交换柱上进行，采用50 mM醋酸钠（pH 5.0）等度洗脱。单-125I-血管紧张素II的纯度通过薄层色谱、高压液相色谱、酶消化、放射受体分析和放射免疫分析来测定。通过该方法制备和纯化单-125I-血管紧张素II在纯度、简便性、效率和成本方面比现有制备方法具有显著优势。