Stadnichuk I N, Idintsova T I, Strongin A Ia
Mol Biol (Mosk). 1984 Mar-Apr;18(2):343-9.
p3phycoerythrin is the major phycobiliprotein of Rhodophyta and endows these algae with the characteristic color. R-phycoerythrin purified from red alga Calithamnion rubosom is composed of four dissimilar polypeptide subunits, alpha, beta, gamma, and delta. In calibrated SDS gel electrophoresis their molecular weights are 21 000, 21 600, 31 000 and 33 000 daltons, respectively. The stoichiometry of the subunits in the native protein is 9 alpha: 9 beta: 2 gamma: 1 delta. R-phycoerythrin carries two covalently linked apoprotein red tetrapyrrol pigments: phycoerythrobilin (PEB) and phycourobilin (PUB). Chemical and spectroscopic data show that alpha subunit carries solely two PEB chromophores, beta subunit--3 PEB and 1 PUB groups, gamma subunit--3 PEB and 2 PUB groups and delta subunit--1 or 2 PEB and 1 PUB groups. The chromophore and polypeptide structure of R-phycoerythrin is mostly composed of all known phycobiliproteins of red and blue-green algae.
藻红蛋白是红藻的主要藻胆蛋白，赋予这些藻类特有的颜色。从红藻Calithamnion rubosom中纯化得到的藻红蛋白由四个不同的多肽亚基α、β、γ和δ组成。在经过校准的SDS凝胶电泳中，它们的分子量分别为21000、21600、31000和33000道尔顿。天然蛋白质中亚基的化学计量比为9α:9β:2γ:1δ。藻红蛋白带有两种共价连接的载脂蛋白红色四吡咯色素：藻红胆素（PEB）和藻尿胆素（PUB）。化学和光谱数据表明，α亚基仅携带两个PEB发色团，β亚基——3个PEB和1个PUB基团，γ亚基——3个PEB和2个PUB基团，δ亚基——1个或2个PEB和1个PUB基团。藻红蛋白的发色团和多肽结构大多由红藻和蓝藻中所有已知的藻胆蛋白组成。
