Cleaver J E
Mutat Res. 1984 Mar-Apr;131(3-4):123-7. doi: 10.1016/0167-8817(84)90051-8.
3-Aminobenzamide is commonly assumed to be a specific inhibitor of poly(ADP-ribose) polymerase. However, it also inhibits de novo synthesis of DNA purines at concentrations of 5 mM and above. A mutant cell line that lacks the ability to incorporate exogenous purines is therefore exceptionally sensitive to the toxic effects of 3-aminobenzamide. This finding has implications for the underlying mechanism of sensitivity to 3-aminobenzamide recently reported in a cell line from an immunodeficient patient, and also implies that many studies using high concentrations of 3-aminobenzamide may not demonstrate a role for poly(ADP-ribose) because of artifacts arising from disturbance of intracellular purine metabolism.
3-氨基苯甲酰胺通常被认为是聚（ADP-核糖）聚合酶的特异性抑制剂。然而，在浓度为5 mM及以上时，它也会抑制DNA嘌呤的从头合成。因此，一种缺乏整合外源性嘌呤能力的突变细胞系对3-氨基苯甲酰胺的毒性作用异常敏感。这一发现对最近报道的来自免疫缺陷患者的细胞系中对3-氨基苯甲酰胺敏感性的潜在机制具有启示意义，也意味着许多使用高浓度3-氨基苯甲酰胺的研究可能无法证明聚（ADP-核糖）的作用，因为细胞内嘌呤代谢紊乱会产生假象。
