Grinvald A, Anglister L, Freeman J A, Hildesheim R, Manker A
Nature. 1984;308(5962):848-50. doi: 10.1038/308848a0.
A major obstacle to understanding the function and development of the vertebrate brain is the difficulty in monitoring dynamic patterns of electrical activity in the millesecond time domain; this has limited investigations of such phenomena as modular organization of functional units, seizure activities and spreading depression. The use of voltage-sensitive dyes and the recent development of the use of an array of photodiodes has provided a unique technique for monitoring the dynamic patterns of electrical activity in real time from a variety of invertebrate or vertebrate neuronal preparations including the rat cortex. In the present study, this technique has been used to investigate the intact optic tectum of the frog. We demonstrate that optical measurements can be used for real-time imaging of spatio-temporal patterns of neuronal responses and for identification of functional units evoked by natural visual stimuli. We report also the structure of the new voltage-sensitive probe that facilitates the in vivo applications of this technique.
理解脊椎动物大脑的功能和发育的一个主要障碍是在毫秒时域内监测电活动动态模式存在困难；这限制了对诸如功能单元的模块化组织、癫痫活动和扩散性抑制等现象的研究。电压敏感染料的使用以及最近光电二极管阵列使用的发展，为实时监测包括大鼠皮层在内的各种无脊椎动物或脊椎动物神经元标本中的电活动动态模式提供了一种独特的技术。在本研究中，该技术已被用于研究青蛙完整的视顶盖。我们证明光学测量可用于神经元反应的时空模式的实时成像以及识别由自然视觉刺激诱发的功能单元。我们还报告了有助于该技术体内应用的新型电压敏感染探针的结构。
