[微生物中游离和结合麦角固醇的测定]
[Determination of free and bound ergosterol in microorganisms].
作者信息
Müller H, Voigt B
出版信息
Z Allg Mikrobiol. 1984;24(1):61-4. doi: 10.1002/jobm.3630240112.
PMID:6719940
Abstract
A method for the quantitative determination of free and esterified ergosterol in lipid fractions from biomasses grown on hydrocarbons as sole carbon source is described. Column chromatography was used for the fractionation of lipids. Esterified ergosterol was determined in the benzene fraction and free ergosterol in the methanol fraction by known methods.
摘要
描述了一种用于定量测定以烃类作为唯一碳源生长的生物质脂质组分中游离和酯化麦角固醇的方法。采用柱色谱法对脂质进行分离。通过已知方法测定苯馏分中的酯化麦角固醇和甲醇馏分中的游离麦角固醇。
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