Bhattacharya P K, Kubinski H, Kubinski Z O
Acta Biochim Pol. 1984;31(1):25-32.
Transcribed portions of mouse nuclear DNA are adjacent to sites reacting preferentially with polyriboguanylic acid [poly(G)]. This spatial relationship was suggested by hybridization of radioactively labeled Ehrlich ascites RNA with denatured mouse DNA, the latter fractionated by centrifugation in CsCl equilibrium density gradients both in the presence and in the absence of various synthetic polyribonucleotides. The fractions of DNA which preferentially reacted with guanylic acid-rich polyribonucleotides hybridized with radioactive RNA to a higher degree than did the bulk of cellular DNA. This ability to react with polyriboguanylic acid-containing polymers was further enhanced by breaking DNA by sonication. No increased tendency for such hybridization was seen with the DNA that preferentially reacted with polyribouridylic acid.
小鼠核DNA的转录部分与优先与聚核糖鸟苷酸[poly(G)]反应的位点相邻。这种空间关系是通过放射性标记的艾氏腹水瘤RNA与变性小鼠DNA杂交提出的，后者在存在和不存在各种合成聚核糖核苷酸的情况下通过氯化铯平衡密度梯度离心进行分级分离。与富含鸟苷酸的聚核糖核苷酸优先反应的DNA部分比大部分细胞DNA与放射性RNA杂交的程度更高。通过超声破碎DNA，与含聚核糖鸟苷酸聚合物反应的能力进一步增强。与聚核糖尿苷酸优先反应的DNA没有观察到这种杂交增加的趋势。
