Lauritsen J G, Therkelsen A J
Acta Obstet Gynecol Scand. 1984;63(1):67-70. doi: 10.3109/00016348409156276.
Using a technique with different media for fertilization and subsequent culture, cleavage of at least one oocyte was achieved in 35 (83%) out of 42 consecutive patients. A total of 138 oocytes (74 pre-ovulatory, 20 non-ovulatory and 44 atretic) were inseminated in vitro. Fifty-two (70%) of the pre-ovulatory oocytes clove and of these 37 (72%) developed apparently normally to the morula or blastocyst stage, while cleavage of the remaining 15 (28%) was characterized by an abnormal growth curve or abnormal morphology of the embryo. Only one (5%) of the 20 non-ovulatory oocytes clove, and this embryo displayed an abnormal growth curve. None of the atretic oocytes clove. It is concluded that evaluation of morphology and growth curve seems to be of value in distinguishing between apparently normally and abnormally developing embryos in vitro.
采用一种使用不同培养基进行受精及后续培养的技术，在连续42例患者中的35例（83%）实现了至少一个卵母细胞的卵裂。总共138个卵母细胞（74个排卵前的、20个未排卵的和44个闭锁的）进行了体外授精。52个（70%）排卵前的卵母细胞发生卵裂，其中37个（72%）明显正常发育至桑葚胚或囊胚阶段，而其余15个（28%）的卵裂特征为胚胎生长曲线异常或形态异常。20个未排卵的卵母细胞中只有1个（5%）发生卵裂，且该胚胎显示生长曲线异常。闭锁的卵母细胞均未发生卵裂。结论是，形态学和生长曲线评估似乎对于在体外区分明显正常发育和异常发育的胚胎具有价值。
