己烯雌酚琼脂糖上雌激素受体的亲和层析

Affinity chromatography of estrogen receptors on diethylstilbestrol-agarose.

作者信息

Van Oosbree T R, Kim U H, Mueller G C

出版信息

Anal Biochem. 1984 Feb;136(2):321-7. doi: 10.1016/0003-2697(84)90224-0.

Abstract

Diethylstilbestrol was coupled to epoxy-activated agarose yielding an affinity resin which is highly efficient for the isolation of estrogen receptors. This resin, diethylstilbestrol-agarose (DES-agarose), bound two proteins (Mr = 50,000 and 65,000) from rabbit uterine cytosol that show a specific interaction with estradiol. A two step procedure--adsorption on DES-agarose followed by a selective elution with p-sec-amylphenol and NaSCN, yielded highly purified estrogen receptors which can be used in the studies of estradiol-receptor interactions with other cell constituents.

摘要

己烯雌酚与环氧活化琼脂糖偶联，得到一种对雌激素受体分离非常有效的亲和树脂。这种树脂，即己烯雌酚-琼脂糖（DES-琼脂糖），能结合来自兔子宫胞质溶胶的两种蛋白质（分子量分别为50,000和65,000），它们与雌二醇表现出特异性相互作用。两步法——先吸附在DES-琼脂糖上，然后用对仲戊基苯酚和硫氰酸钠进行选择性洗脱，得到了高度纯化的雌激素受体，可用于研究雌二醇-受体与其他细胞成分的相互作用。