用于纯化雌激素受体的可溶性生物特异性大分子。
Soluble biospecific macromolecule for purification of estrogen receptor.
作者信息
Hubert P, Mester J, Dellacherie E, Neel J, Baulieu E E
出版信息
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3143-7. doi: 10.1073/pnas.75.7.3143.
Abstract
An alternative to affinity chromatography purification of proteins based on the use of a water-soluble biospecific polymer has been applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (molecular weight approximately 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released from the dextran-estradiol conjugate by exchange with [3H]estradiol.
摘要
一种基于使用水溶性生物特异性聚合物的蛋白质亲和层析纯化替代方法已应用于小牛子宫的雌激素受体。该受体（4S-胰蛋白酶形式）与葡聚糖-雌二醇缀合物（分子量约500,000）结合，然后通过凝胶过滤分离复合物。随后通过与[3H]雌二醇交换，从葡聚糖-雌二醇缀合物中释放出高度纯化的受体。
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