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用于纯化雌激素受体的可溶性生物特异性大分子。

Soluble biospecific macromolecule for purification of estrogen receptor.

作者信息

Hubert P, Mester J, Dellacherie E, Neel J, Baulieu E E

出版信息

Proc Natl Acad Sci U S A. 1978 Jul;75(7):3143-7. doi: 10.1073/pnas.75.7.3143.

DOI:10.1073/pnas.75.7.3143
PMID:277916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392730/
Abstract

An alternative to affinity chromatography purification of proteins based on the use of a water-soluble biospecific polymer has been applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (molecular weight approximately 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released from the dextran-estradiol conjugate by exchange with [3H]estradiol.

摘要

一种基于使用水溶性生物特异性聚合物的蛋白质亲和层析纯化替代方法已应用于小牛子宫的雌激素受体。该受体（4S-胰蛋白酶形式）与葡聚糖-雌二醇缀合物（分子量约500,000）结合，然后通过凝胶过滤分离复合物。随后通过与[3H]雌二醇交换，从葡聚糖-雌二醇缀合物中释放出高度纯化的受体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ab4/392730/ae05b7cdfe93/pnas00019-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ab4/392730/ae05b7cdfe93/pnas00019-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ab4/392730/ae05b7cdfe93/pnas00019-0142-a.jpg

相似文献

1
Soluble biospecific macromolecule for purification of estrogen receptor.用于纯化雌激素受体的可溶性生物特异性大分子。
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3143-7. doi: 10.1073/pnas.75.7.3143.
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Estrogen-binding proteins of calf uterus. Purification to homogeneity of receptor from cytosol by affinity chromatography.小牛子宫的雌激素结合蛋白。通过亲和色谱法从胞质溶胶中纯化受体至同质状态。
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Parameters influencing the purification of calf uterus estrogen 'receptor' by affinity chromatography.影响通过亲和层析法纯化小牛子宫雌激素“受体”的参数。
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本文引用的文献

1
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
2
Human erythrocyte pyruvate kinase. Its purification and some properties.人红细胞丙酮酸激酶。其纯化及某些性质
Biochim Biophys Acta. 1971 Jan 13;227(1):86-96. doi: 10.1016/0005-2744(71)90170-7.
3
Determination of high-affinity oestrogen receptor sites in uterine supernatant preparations.子宫上清液制剂中高亲和力雌激素受体位点的测定。
Biochem J. 1970 Dec;120(4):831-6. doi: 10.1042/bj1200831.
4
Criteria for affinity chromatography of steroid-binding macromolecules.类固醇结合大分子亲和层析的标准。
J Biol Chem. 1972 Dec 10;247(23):7533-8.
5
Affinity chromatography.亲和层析
Annu Rev Biochem. 1971;40:259-78. doi: 10.1146/annurev.bi.40.070171.001355.
6
Purification and allosteric properties of yeast pyruvate kinase.酵母丙酮酸激酶的纯化及其别构性质
Hoppe Seylers Z Physiol Chem. 1968 May;349(5):699-714. doi: 10.1515/bchm2.1968.349.1.699.
7
Parameters influencing the purification of calf uterus estrogen 'receptor' by affinity chromatography.影响通过亲和层析法纯化小牛子宫雌激素“受体”的参数。
FEBS Lett. 1974 Sep 15;46(1):321-5. doi: 10.1016/0014-5793(74)80397-2.
8
Affinity chromatography and the purification of estrogen receptors.亲和层析与雌激素受体的纯化
J Biol Chem. 1973 Sep 25;248(18):6543-58.
9
Affinity chromatography of the uterine estradiol receptor on estradiol-PAB-cellulose: an artefact.子宫雌二醇受体在雌二醇-PAB-纤维素上的亲和层析：一种人为现象。
Biochimie. 1976;58(7):863-9. doi: 10.1016/s0300-9084(76)80317-3.
10
Production and detection of antibodies against the estrogen receptor from calf uterine cytosol.来自小牛子宫胞质溶胶的抗雌激素受体抗体的产生与检测。
FEBS Lett. 1976 Mar 15;63(1):71-6. doi: 10.1016/0014-5793(76)80197-4.