Wagner H J, Schülke B
Biomed Biochim Acta. 1984;43(1):3-9.
Mammalian tissues contain cytosolic and microsomal glucose 6-phosphate dehydrogenase (G6PD). In the present study it was examined whether the microsomal G6PD in liver and testis of the rat are homologous. Kinetic parameters and immunological reactivity were estimated using rabbit antiserum against mice erythrocyte G6PD. Kinetic parameters allow to distinguish cytosolic and microsomal G6PD in rat liver but not in testis, whereas the immunological properties of the testis enzymes are different. The cytosolic G6PDs from liver and testis of the rat are identical enzymes having extremely similar properties to mice erythrocyte G6PD. The microsomal G6PD of both organs are not homologous enzymes. Testicular microsomal G6PD is likely to be an organ specific G6PD-isoenzyme.
哺乳动物组织含有胞质和微粒体葡萄糖-6-磷酸脱氢酶（G6PD）。在本研究中，检测了大鼠肝脏和睾丸中的微粒体G6PD是否同源。使用抗小鼠红细胞G6PD的兔抗血清评估动力学参数和免疫反应性。动力学参数能够区分大鼠肝脏中的胞质和微粒体G6PD，但不能区分睾丸中的，而睾丸酶的免疫特性不同。大鼠肝脏和睾丸中的胞质G6PD是相同的酶，其性质与小鼠红细胞G6PD极其相似。两个器官的微粒体G6PD不是同源酶。睾丸微粒体G6PD可能是一种器官特异性G6PD同工酶。
