Gains N, Hauser H
Biochim Biophys Acta. 1984 May 16;772(2):161-6. doi: 10.1016/0005-2736(84)90039-7.
From the water content of pelleted brush-border vesicles and from a comparison of the aqueous volume within the pellet that is available to [3H]inulin (58%), inulin [14C]carboxylic acid (34%, both approx. 5000 daltons), [3H]raffinose (97%, 540 daltons) and [3H]glucose (94%, 180 daltons) it is concluded that only 1 in 4 to 6 of the brush-border vesicles is sealed. The implication of this finding for labelling and transport studies and for vesicle formation is discussed.
根据颗粒状刷状缘小泡的含水量,以及对颗粒内可被[3H]菊粉(58%)、菊粉[14C]羧酸(34%,两者分子量均约为5000道尔顿)、[3H]棉子糖(97%,540道尔顿)和[3H]葡萄糖(94%,180道尔顿)利用的水相体积进行比较,得出结论:只有四分之一到六分之一的刷状缘小泡是封闭的。文中讨论了这一发现对标记和转运研究以及小泡形成的意义。